Research Lab

Research projects, past and present, under N. Rojas

1. Searching for diagnostic and prognostic markers in urinary proteins using 2-D electrophoresis
   1. Mailyn M. Terrado, M.S. Chemistry candidate
   2. Coratet Fernandez, BS Chemistry 2010
   3. Steven J. Chuacokiong, B.S. Chemistry 2009 (now B.S. MSE)
   4. Cesare Mikhail R. Cejas, B.S. Chemistry 2008, BPI Science Awardee (now at the SERPInternational Masters Program– France-Poland-Italy)
2. Proteins associated with carrageenan and carrageenan-producing algae
   1. Paulina S. Aspilla, Ph.D. candidate
      1. Isolation and characterization of the major soluble protein from Eucheuma denticulatum (iota-carrageenan producing seaweed)
      2. cDNAs from Eucheuma denticulatum
   2. Yasmin Chan, BS Chemistry 2010
   3. Anna Angela Camille B. Antonio, B.S. Chemistry 2009: Partial designation of expressed sequence tags from Eucheuma denticulatum
   4. Bernadette M. Henares, M.S. Chemistry 2007 (now a Ph.D. student at Stony Brook University (State University of New York): Iota-carrageenase activity of Pseudoalteromonas carrageenovora
   5. Andreneve L. Tan (B.S. Chemistry 2008, B.S. MSE 2009): Primer design for the iota-carrageenase gene from P. carrageenovora
3. Glycosidases
   1. Ginger glycosidases
      1. David P. Peralta, M.S. Chemistry 2009, B.S. Chemistry, BPI Science Awardee  (now a Ph.D. student at the German Cancer Research Institute or DKFZ): A 31.8 kDa protein from ginger rhizome (Zingiber officinale Roscoe) that hydrolyzes beta-glucosides.
      2. Christine Ann B. Tolentino, B.S. Chemistry 2004. Isolation of glycosidases from different types of Zingiber officinale Roscoe.
      3. Maria Elena R. Tongol, B.S. Chemistry 2003. Isolation and substrate selectivity of glycosidases in Zingiber officinale Roscoe.
   2. Garlic
      1. Mailyn M. Terrado, B.S. Chemistry 2003. Isolation and substrate selectivity of glycosidases in Allium sativum L.
   3. General screening for glycosidases: Abigail T. Go, B.S. Chemistry 2001
4. Using and testing bioinformatics tools
   1. Joselito M. Lagazo, B.S. Chemistry 2006. Mapping structural and functional aspects of the human cysteinyl leukotriene receptor 1.
   2. Katherine S. Ong, B.S. Chemistry 2005. Homology modeling and hypothetical mapping of the active site of human 5-lipoxygenase using public access tools.
   3. Frances C. Vega, B.S. Chemistry 2005, BPI Science Awardee; Master in Chemistry 2007, M.A. Political Science/International Relations: Sequence analysis of mucins
   4. Paul Dominic B. Olinares, B.S. Chemistry 2002
   5. Abigail T. Go, B.S. Chemistry 2001, (later Ph.D. Chemistry, Princeton University, 2008): An online bioinformatics guide to ExPASy tools for plant proteomics <http://www.chem.admu.edu.ph/~nina/abi/>
   6. Paul Rosby M. Quiambao, B.S. Chemistry 2000, B.S. Computer Engineering/Applied Computer Systems 2001. a tutorial for protein structural analysis using internet tools <http://www.chem.admu.edu.ph/~nina/rosby/>
5. Protein lab tools
   1. Molecular weight analysis of proteins using electrospray ionization mass spectrometry:
      1. Faith M. Justimbaste, B.S. Chemistry 2004.
      2. Lieza MarieA. Danan, B.S Chemistry 2001.
      3. Mark Percival O. de Guzman, B.S. Chemistry 2002, B.S. Computer Engineering/Applied Computer Systems 2003. Resolving peak multiplicity using the averaging method and Labview(TM).
   2. 2-D electrophoresis
      1. Mija Darlene A. Cu, B.S. Chemistry 2002, B.S. Computer Engineering/Applied Computer Systems 2003. Expanded protein profiling by enhanced solubilization of hydrophobic proteins.
      2. Niña Socorro D. Cortina, B.S. Chemistry 2001, B.S. Computer Engineering/Applied Computer Systems 2002 (later M.S. Chemistry 2006-Ateneo sandwich and Ph.D. student at Saarland University, Germany): Proteome analysis of Vitex negundo.
      3. Bernadette Henares: Protein profiling of garlic
   3. Enzyme immobilization: Ma. Katrina Macias, B.S. Chemistry 1999, B.S. Computer Engineering/Applied Computer Systems 2000. Immobilization of horseradish peroxidase
6. Expression of scFv in E. coli under the Antibody-based Molecular Oncology Research Project
   1. Mailyn M.Terrado
   2. Bernadette M. Henares
   3. Paul Dominic B. Olinares, B.S. Chemistry 2002, College Valedictorian, BPI Awardee (now Ph.D. student at Cornell University)
   4. Mark Noel F. Mabanglo, B.S. Chemistry 2002 (now Ph.D. student at University of Utah)
7. Natural products
   1. Moira Cruz, B.S. Chemistry 1998: HPLC analysis of vinbastin from chichirica plants.